hydrogen rich saline

Therefore HS may be used as a manageable safe and portable clinical hydrogen delivery method. Normal saline NS HRS lipopolysaccharide LPS and LPS plus HRS.

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The saturated hydrogen saline was stored under atmospheric pressure at 4C in an aluminum bag with no dead volume.

. Hydrogen-Rich Saline Production Hydrogen was dissolved in physiologic saline 6 h under high pressure 04 MPa to a supersaturated level using hydrogen-rich saline-producing apparatus produced by our department. 1Morphological study showed after 90 min of intestinal ischemia and 4 h of reperfusion the lung tissues of rats were severely damaged in the saline-treated group with severe edema severe alveolar hemorrhage and extensive. A number of studies have shown that hydrogen-rich saline HRS has a beneficial effect on sepsis.

In addition the present study revealed that H2rich saline could significantly increase peroxisome proliferatoractivated receptor PPAR α and PPARγ expression in hepatocytes. Hydrogen-rich saline protects against acute lung injury induced by extensive burn in rat model Abstract Hydrogen has been reported to selectively quench detrimental reactive oxygen species particularly hydroxyl radical and to prevent myocardial or hepatic ischemiareperfusion injury in multiple models. IR1 NS group one group treated with 20 min of ischemia and HS IR1 HS group one group treated with 60 min.

However it is unknown whether these effects involve autophagy induction. It was ensured that hydrogen-rich saline was maintained at a concentration of more than 06 mmoll. Four groups of rats received intraperitoneal injections of one of the following solutions.

Hydrogen-rich saline HRS has antioxidative anti-inflammatory and anti-apoptotic properties. Hydrogen was dissolved in normal saline under the pressure of 05 MPa for 7 hours to reach the saturation by using the apparatus Hydrovita Biotechnology Co Beijing China. The use of saline in therapeutic doses of hydrogen hydrogen-rich saline HS is a technique used to transfer molecular hydrogen from blood to the nucleus and mitochondria of cells 717.

Hydrogen-rich saline solution and intestinal anastomosis In Animal studies Gut by CHESSJuly 11 2021 The authors investigated the effects of hydrogen-rich saline solution HRSS on intestinal anastomosis performed after intestinal ischemia reperfusion injury IRI. The hydrogen concentration was detected by gas chromatography using the method previously described by Ohsawa et al 22. Hydrogen-rich saline was freshly prepared each week and was sterilized by g radiation.

Hydrogen-rich saline is an alternative form of molecular hydrogen that has been widely used in many studies including metabolic syndrome cerebral hepatic myocardial ischemiareperfusion and liver injuries with obstructive jaundice with beneficial results. Therefore we tested whether HRS prevents cardiac dysfunction by increasing cardiac energy. The hydrogen level in the saline was measured using gas chromatography as described by Ohsawa et al6The average hydrogen level was 085 mmolL.

Hydrogen-rich saline was stored under an atmospheric pressure at 4C in an aluminum bag with no dead volume sterilized by gamma radiation which was freshly prepared once a week to ensure that the concentration was maintained at 06 mmoll. Rats received intraperitoneal hydrogen-rich saline at 10 mlkg body weight for 14 days. Forty rats were randomly allocated into five groups.

We investigated the relationship between HRS and cell autophagy in a neuropathic pain model generated by chronic constriction injury CCI in SpragueDawley rats. Recently the US Food and Drug Administration acknowledged hydrogen H 2 gas as food additives when used in drinking water or beverages and declared them to be generally recognized as safe. Hydrogen-rich saline was freshly prepared every week to ensure that the concentration.

Thirty Wistar albino female rats were randomly divided into five groups. Twenty-one healthy Bama miniature pigs were randomly divided into the sham group ischemia-reperfusion injury IRI group HRS-5 5 mLkg. Cell proliferation inhibition assay.

Hydrogen-rich saline regulates cisplatin-induced oxidative stress by increasing the activity of SOD and CAT and reducing the level of MDA in the serum and ovarian tissue of rats. Cisplatin was injected at 5 mgkg in group OI. More importantly a higher concentration of hydrogen can be dissolved into HRS 28.

Hydrogen-rich saline HRS reportedly reduces allodynia and hyperalgesia in a neuropathic pain model. The effects of hydrogen-rich saline treatment on the histopathological changes of lungs in rats with intestinal IR were shown in Fig. In short H 2 gas 04 mPa was dissolved in normal saline for at least 2 hours to reach supersaturated level 06 mmolL.

One sham group control group one group treated with 20 min of ischemia and normal saline NS. Hydrogen-rich saline alleviates inflammation and apoptosis in myocardial IR injury via PINK-mediated autophagy Ischemiareperfusion IRinduced inflammatory reaction is one of the most important elements in myocardial IR injury. Hydrogen can be dissolved in water up to 08 mM under atmospheric pressure at room temperature and its solubilized form the hydrogen-rich saline HRS is advantageous since it is a safe portable and easily handled approach for delivery.

Department of Physiology School of Basic Medical Sciences Cheeloo College of Medicine Shandong University Jinan Shandong 250012 Peoples Republic of China Email wangzhensdueducn Introduction. To evaluate the effect of hydrogen-rich saline HS on hepatic ischemia-reperfusion IR injury. Hydrogen-rich saline inhibits tobacco smoke-induced chronic obstructive pulmonary disease by alleviating airway inflammation and mucus hypersecretion in rats Source.

In conclusion H2rich saline may significantly improve NAFLD possibly by reducing oxidative stress and activating hepatic PPARα and PPARγ expression. Then the saturated HRS was stored under atmospheric pressure at 4C and then sterilized with γ. We investigated the effects of hydrogen on hepatic ischemia-reperfusion IR and laparoscopic hepatectomy in swine.

We have reported previously that hydrogen-rich saline HS plays a neuroprotective role in hypoxia-ischemia HI brain damage in newborn mice. Hydrogen-rich saline promotes microglia M2 polarization and complement-mediated synapse loss to restore behavioral deficits following hypoxia-ischemic in neonatal mice via AMPK activation These results demonstrate that HS restored behavioral deficits. Hydrogen-rich saline was generously offered by Professor Xuejun Sun the Second Military Medical University Shanghai China and prepared to confirm the content of hydrogen in saline as described previously 16.

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